Peptide reconstitution calculator
Enter your vial size, bacteriostatic water volume, and target dose. The calculator returns concentration, the volume to draw, and units on a U-100 insulin syringe.
Math, not medical advice. Verify against the vial label and your protocol before drawing.
Reference
Short notes on the terminology behind the inputs above. Skip if you already know.
What is bacteriostatic water, and what is the 28-day rule?
Bacteriostatic water is sterile water with about 0.9 percent benzyl alcohol added as a preservative. It’s the diluent most commonly used to reconstitute lyophilized peptides because the preservative inhibits microbial growth long enough to use the vial across multiple injections.
The 28-day rule is the manufacturer-stated stability window once the preservative has been introduced — after 28 days, the bacteriostatic protection is no longer assumed to hold. Joust tracks this per-vial as the BUD (beyond-use date) and warns you before it elapses.
U-100 vs U-40 — which syringe do I use?
Insulin syringes are calibrated in units. U-100 means 100 units per millilitre; U-40 means 40 units per millilitre. The same volume of fluid reads a different unit number depending on which syringe you’re looking at, so the choice matters when you’re converting a target dose into a number to draw to.
U-100 (red cap, in most regions) is the more common choice for peptide work and matches what most pharmacies stock. U-40 (yellow cap) is more common in veterinary contexts and in regions where U-40 insulins are still used. Pick whichever one matches the physical syringe in front of you.
How does concentration affect doses per vial?
Total mg in the vial is fixed by the manufacturer — adding more or less bacteriostatic water doesn’t change how much active is in the vial, only how that mass is distributed across volume. Reconstituting a 10 mg vial with 1 mL gives 10 mg/mL; the same vial reconstituted with 2 mL gives 5 mg/mL.
More water means a lower concentration, which means each dose takes up a larger volume — and a larger number of units on the syringe. Doses-per-vial stays the same regardless: a 10 mg vial at 1 mg per injection yields ten injections either way. People choose more water when they want easier-to-read draw amounts and less when they want fewer units per injection.
How reconstitution math actually works.
A short, careful walkthrough — concentration, volume, units — written so you can verify your own numbers. Publishing soon.